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Amaxa cell line nucleofector kit v
Cell Line Nucleofector Kit V, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+nucleofector+kit+v/electroporation/pmc13144684-321-17-16
Average 86 stars, based on 1 article reviews
cell line nucleofector kit v - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Transfection:

Article Title: TNFAIP3, PADI4, and CXCR4 as biomarkers of neutrophil extracellular traps in severe asthma.
Article Snippet: Asthma is a heterogeneous condition impacting an estimated 300 million individuals globally.. Although inhaled corticosteroids are effective in alleviating asthma symptoms, approximately 22 % 60 % of patients still experience poor disease control.. Among these patients, the majority exhibit abnormalities in neutrophils.

Article Title: A prime editing strategy to rewrite the γ-globin promoters and reactivate fetal hemoglobin for sickle cell disease
Article Snippet: .. Briefly, K562 cells were grown and transfected with plasmids using the Amaxa Cell Line Nucleofector Kit V and U-16 program (Lonza)., HSPC collection, culture and PE/BE transfection were performed as described in prior studies , and in the legends of and . .. Genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Invitrogen).

Article Title: ST3GAL3-mediated selective α2,3-sialylation of LDLR promotes vesicular stomatitis virus entry.
Article Snippet: Background: Vesicular stomatitis virus (VSV) is a model rhabdovirus whose infectivity is determined primarily by its envelope glycoprotein (VSV-G).. The low-density lipoprotein receptor (LDLR), a cell-surface glycoprotein, has been identified as the major receptor for VSV-G binding.. However, the role of host sialylation in VSV-G-dependent entry remains poorly understood.

Article Title: Association of fibronectin 1 deregulation with tyrosine kinase inhibitor resistance in chronic myeloid leukemia
Article Snippet: Fluorescent cells binding to Matrigel were measured at an Infinite M200 Pro device (Tecan Group, Crailsheim, Germany) with 494 nm absorbance and 517 nm emission. .. Transient transfection was either performed using the nucleofector 2b device and the Amaxa Cell Line Nucleofector Kit V or the nucleofector 4D device and the SF Cell Line 4D Nucleofector Kit X (all Lonza, Basel, Switzerland). .. 3–5 × 10 6 K-562 or Ba/F3 cells were transfected with 5 or 2 μg of the respective plasmid (pSELECT-FN1) or empty vector control (InvivoGen, Toulouse, France).

other:

Article Title: Compositions and methods for the diagnosis and treatment of retinopathies
Article Snippet: The tagged constructs were cloned into the pCAG-YFP plasmid (Addgene #11180).

Plasmid Preparation:

Article Title: Androgen receptor localisation and protein interactions provide insight into steroid mediated metabolic shifts in endocrine resistant breast cancer
Article Snippet: .. Nucleofection (Lonza nucleofector 2b) was used to transfect the plasmid into the cells following protocol from Amaxa® Cell Line Nucleofector® Kit V. This is a technique which uses electrical pulses to create small pores in the cell membrane in order to enhance plasmid entry into the cells. .. Cells were harvested by trypsinization and counted using countess automated cell counter (Invitrogen).

Membrane:

Article Title: Androgen receptor localisation and protein interactions provide insight into steroid mediated metabolic shifts in endocrine resistant breast cancer
Article Snippet: .. Nucleofection (Lonza nucleofector 2b) was used to transfect the plasmid into the cells following protocol from Amaxa® Cell Line Nucleofector® Kit V. This is a technique which uses electrical pulses to create small pores in the cell membrane in order to enhance plasmid entry into the cells. .. Cells were harvested by trypsinization and counted using countess automated cell counter (Invitrogen).



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https://www.bioz.com/product/cell+line+nucleofector+kit+v/electroporation/pmc13144684-321-17-16
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